The Changes Of NF-κB And TNF-α In Diabetes Mellitus Rat's Myocardium Following Limbs Ischemic Reperfusion And The Intervention Effects Of Propofol

Objective To investigate the mechanism of action of cytokine and nuclear factor in normal rats and diabetes mellitus rats following limbs ischemic reperfusion and the mechanism of protect action of Propofol, the experiment was made on the changes of nuclear factor of kappa B ( NF-κB) and tumor nerosis factor-α(TNF-α)in diabetes mellitus rat's myocardium following limbs ischemic reperfusion and the effect of Propofol in the process.Method Adult male Ssprague-Dawley rats, weighing 220-250g were randomized assigned to normal rats and diabetes mellitus rats. The rats were induced diabetes mellitus with streptozotocin (STZ).The normal rats were divided into three groups at random: group IA(n=8) control group,ⅠB(n=8) limbs ischemic reperfusion group , groupⅠC(n=8) limbs ischemic reperfusion and Propofol group. Diabetes mellitus rats were divided into four group at random: groupⅡA(n=8) control group, groupⅡB(n=8) limbs ischemic reperfusion group, groupⅡC(n=8) Propofol group, groupⅡD(n=8) limbs ischemic reperfusion and Propofol group.the exprement deployed immunohistochemistry(IHC) in order to detect qualitatively and semiquantitatively the express of TNF-αand NF-κB.and radio-immunity(RI) in order to detect quantitative the level of TNF-α.Result First,limbs ischemic reperfusion(group IB)can cause myocardial damage.compared with normal rat( group IA), the level of NF-κB and TNF-αis increase and there is statistical significance (P<0.05). Second, diabetes mellitus rats(groupⅡA)can cause myocardial damage.compared with normal rat( group IA), the level of NF-κB and TNF-αis increase and there is statistical significance (P<0.05). Third, diabetes mellitus with limbs ischemic reperfusion can cause myocardial significant damage, compared with normal rat(group IA ) and limbs ischemic reperfusion(groupⅠB)and diabetes mellitus rats(groupⅡA),the level of NF-κB and TNF-αis predominance heighten. and there is statistical significance (P<0.05). Fourth, compared with theAP:PA of intervention of Propofol ,every group level of NF-κB and TNF-αis cut down after intervention of Propofol and there are statistical significance (P<0.05). Fifth,the rest is not significant difference each other (P>0.05).Conclusion First, the level of NF-κB and TNF-αwas step up for diabetes mellitus rat and limbs ischemic reperfusion rat with diabetes mellitus and limbs ischemic reperfusion rat without diabetes mellitus. Moreover, the level is maxlmum for diabetes mellitus with limbs ischemic reperfusion rat. Second, Propofol can cut down the level of NF-κB and TNF-αfor diabetes...