Role Of Nuclear Factor-κB In Pathogenesis Of Endometriosis

Endometriosis is a reproductive disease characterized the presence and growth of ectopic endometrial tissue at sites outside the uterus. With the incidence in the general population gradually being raised in the past few years, Endometriosis has been considered to be one of the most commonly encountered diseases of gynecology besides myoma of uterus. Endometriosis is common benign condition, though its pattern of growth is similar to malignant disease. Endometriosis still remains controversial despite extensive research; thus it is difficult to be treated with surgery or medicine. The rate of 5-year-recurrence is above 40% even by diverse the agents. It is now generally accepted that the pathogenesis of endometriosis involves the implantation of exfoliated endometrium. Recent reports have shown that endometriosis is an inflammatory-associated disease and pelvic inflammation may be involved in the pathogenesis or progression of endometriosis.Nuclear factor-кB (NF-кB) represents an important group of regulators of a broad range of genes involved in cellular responses to immunity, nflammatory and stress signals. NF-кB is one of the pivotal regulators of proinflammatory gene expression and induces transcription of proinflammatory cytokines (like TNF-α, IL-1, IL-6, IL-8 ), adhesion molecules, chemokines. NF-кB plays a crucial role in a variety of inflammatory diseases such as asthma, rheumatoid arthritis, inflammatory bowel diseases and cancer. Currently, there are not systematic studies on the role of NF-кB in the pathogenesis of endometriosis. In this study, our purpose is (ⅰ) to explore the isolation and culture of ectopic and eutopic endometriotic glandular epithelial cell and its stromal cell ; and(ⅱ)to evaluate the effect of TNF-αon proliferation of ectopic and eutopic endometrial stromal cells, NF-кB activation and release of IL-8 by the same endometrial stromal cells in culture. These results will not only demonstrate NF-кB plays an important role in the pathogenesis of endometriosis, but also provide novel therapeutic modalities for endometriosis in the near future.Part I:Isolation and culture of ectopic and eutopic endometrial glandular epithelial cells and stromal cellsObjective: To explore the isolation and culture of ectopic and eutopic endometrial glandular epithelial cell and its stromal cell. Methods: Enzymolysis, serial filtration, transfer, and pasted wall purification were used to isolate, purify and culture in 20 samples of ectopic and eutopic endometrium from patients with endometriosis and 20 samples of endometrium from patients without endometriosis. Results: The success cutured rate of endometrium from patients with endometriosis and without endometriosis were significantly increased, as compared with ectopic...