The Protein Expressive Tendency Of Plasminogen Activators(PAs) And 1-type Plasminogen Inhibitor(PAI-1) Remnant Renal Tissues Of 5/6 Nephrectomy Young Rats And The Effects Of Interfering Treatment

Objective: To investigate the changes of fibrinolysis system during progressive chronic renal lesions or tubulointerstitial fibrosis in remnant renal tissues of 5/6 Nephrectomy young rats,and to evaluate the correlation between of them, and to study the interfering effects of treated with angiotensin converting enzyme inhibitor(ACEI) Benazepril and Hirudo.Methods: 5/6 Nephrectomy remnant young rats used as a classical chronic renal failure model. When the models were succeed that through evaluated the proteinuria, blood pressure, serum bichemical parameters and renal pathological changes. Then, those model animals were divided randomly into five groups. That was 5/6NX untreated group; 5/6NX treated with Hirudo; 5/6NX treated with ACEI; 5/6NX treated by ACEI combined with Hirudo and sham group. At begin in the experiment, 4th week, 8th week, and 12th week, we measured the body weight, blood pressure and proteinuria in 24 hours of rats, and detected the protein expression of PAI-1, uPA and tPA in remnant renal tissues by the way of immunohistochemical technique. These protein expressing changes and relativity of them were evaluated and to analysis the pathogenic role of these parameters changes in renal lesions, and to visit the interfering effects of treatment with angiotensin converting enzyme inhibitor(ACEI) Benazepril and thrombin inhibitor-Hirudo.Results: The renal failure was observed progressively after model young rats undergoing 5/6 Nephrectomy in untreated group. At the 12th week followed treatment, the body weight of rats were lose step by step, and blood pressure, urinary protein were increased progressively. At all time points in experiment, each serum bichemical parameters reflecting renal function are normality in sham group rats. However, the BUN, Scr etc. were deteriorated progressively in 5/6NX untreated group rats, and their plasm total protein and albumin were decreased progressively. The hyperlipemia and chronic kidney failure were presented in those model rats. But in each treated group, these changes were ameliorated following treated with Hirudo and ACEI or combined with two medicines. This treated effect was more excellent in combining...