| Objective: The transplantable tumor animal model may objectively simulate the process of growth in human tumor. Accordingly, it was generally applied to the research of the tumorigenic mechanism and the screening of the anti-tumor drugs. In the long process of passage, tumor cell strains are exposed to variety of unstable factors, such as genetic pollution and genovariation. Therefore, through a long time it is possible that variation of genetic mass and the following changes in biological characteristics may decrease the homogenicity and comparison of tumor animal models. If wanting to keep the stability of the animal models of transplanted tumors, we have to give quality control on the tumor cell strains.Hepatoma 22(H22) is one of the most usually used mice transplanted tumor cell lines and widely applied to make the mouse tumor models. This cell line has been set up for nearly 50 years. Many scholars reported that it had already undergone biggish genetic variance and the cell strains conserved in different institutes had been found large difference. Liu junxu etc have made H22 cloning and got 4 clone strains, including H22-H8D8,H22-H2C4,H22-H2G4,H22-H2E10.Herein...
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