Effects Of CRE-decoy ODN On The Signal Molecule Concerned In Morphine Dependent SK-N-SH Cells

Objective: Drug dependence is a chronic, relapsing disease, characterized by compulsive drug caving and use and by neurochemical and molecular changes in the brain. The powerful and uncontrollable desire for drugs often makes the addict use the drug continually regardless of the damage to their body. In our country the drug of abuse is mostly the material of opioid type. The therapy of drug dependence is not only involving the treatment to substitute and symptom, but also involveing the adaptability changes of other neurotransmitters and their signal transudations. The cAMP, as important second messengers play a vital role in producing drug tolerance and dependence. In the central nervous chronic morphine treatment leads to an up regulation of cAMP production and causing phosphorylation of CREB by cAMP-dependence protein kinas A(PKA).These results raise the possibility that CREB activation during morphine withdrawal may up-regulate CREB-regulated target genes, and lead to the nerve cell change of plasticity and adaptability, which is the substance base of drug dependence. The CRE cis-element is palindrome and the CRE-transcription factor complex is a pleiotropic activator, which participates in the induction of a wide variety of cellular and viral genes. In this...