The Experimental Study Of Olfactory Ensheathing Cells Transplantation Therapy In Cortical Infarction

Background and ObjectiveTraumatic injuries such as ischemia to the adult mammalian central nervous system (CNS) cause severe disabilities, because of the loss of large number of neurons in the core of infarct. Some researches suggest the involvement of axonal sprouting followed by synaptogenesis in the neocortex after focal ischemia as a mechanism for function recovery. Some important factors for lack of regeneration in the central nervous system after traumatic injuries are the extensively existent of nerve growth inhibitors and gliosis.Olfactory ensheathing cells (OECs) are very unique cells and hypothesized to facilitate regeneration of central nervous system and excreting neurotrophic factors.It has been proved that olfactory ensheathing cells (OECs) transplantation on the spinal cord injuries can promote the regeneration of spinal cord axons and functional recovery, but there was no research on the OECs transplantation in cerebral infarction. Our experiment establishes a simple and efficiency method for OECs culture and purification , OECs survival and repair in vivo for cerebral infarction after the OECs transplantation were also observed.