Study On The Protective Effect Of Radiation Damage And Enhancing Efficacy Of Radio-Therapy With ABP On Tumor-Bearing Mice

Objective: To study the protective effect of radiation damage and enhancing efficacy of radiotherapy with ABP on tumor-bearing mice. Methods: The H₂₂ tumor-bearing mice were treated with 5.0Gy ⁶⁰Coγ after administered with ABP i.p. The number of leucocytes in peripheral blood, indexes of thymus and spleen and level of DNA in bone marrow were detected after radiation to those mice. Activity of SOD and Content of MDA in serum was determined. Flow cytometric analysis was used to determine the apoptotic tumor cells;Electron microscope was applied to observe the morphological change of tumor cell treated with ABP. Results: The results showed that ABP markedly lessened the descent in number of leucocytes and spleen and thymus weigh caused by radiation. ABP resulted in a drop in level of MDA in serum. ABP increased in the level of SOD in serum and DNA in bone marrow. ABP enhanced inhibited effect on H₂₂ tumor by radiotherapy for three different dosages. It showed that low dosage group had the significant inhibited effect on tumor with the inhibitive rate of 64.42%. Flow cytomertric analysis revealed that 13.80% apoptotic cells were seen in the group treated with ABP combined with radiotherapy while there were 4.27% apoptotic cells in alone radiotherapy. Conclusion: ABP has protective effect on radiation damage of tumor-bearing mice. ABP can markedly promote the inhibition effect on H₂₂ tumor with radiotherapy.