Application Of Chemiluminescence In Pharmaceutical Analysis

The review involves the the history, the basic principles, characteristics and the main systems of chemiluminescence (CL), and the applications of CL systems in pharmaceutical analysis in last five years are intensively reviewed.The research report focused on the CL system with ClO₂ as oxidiant and the second CL emission in luminol-Fe(CN)6 ^3- -caffeine system.1. Homogeneous chemiluminescence system in neutral and near neutral aqueous solution with ClO₂ as oxidant and its analytical application.Using ClO₂ as chemiluminescence (CL) oxidant, a novel homogeneous CL system in neutral and near neutral aqueous solution is proposed in this paper. ClO₂ could oxidize sulfite in pH 5.0-8.5 acetate buffer to produce CL emission, and pipemidic acid could sensitize the CL system. The C102-S03^2--pipemidic acid reaction was used as a model CL system and explored the possibility of highly active and eco-friendly ClO₂ being used as CL oxidant in neutral and near neutral aqueous solution. Combined with flow-injection analysis, the proposed CL system was applied to the automated dissolution testing of pipemidic acid tablet. Compared with the common strong acidic or strong basic CL system, this proposed CL owns its advantages.2. Chemiluminescence system in unbuffer aqueous solution with ClO₂ as oxidant and its analytical application.Using ClO₂ as chemiluminescence (CL) oxidant, a novel homogeneous CL system in unbuffer aqueous solution is proposed in this paper. ClO₂ could oxidize sulfite in unbuffer aqueous solution to produce CL emission, and VB₂ could sensitize the CL system. The ClO₂-SO₃^2--VB₂ reaction was used as a model CL system and explored the possibility of ClO₂ being used as CL oxidant in unbuffer aqueous solution. Combined with flow- injection analysis, the proposed CL system was applied to determine VB₂ in tablets and injection samples with satisfactory results. Compared with other similar energy-transfer CL system, this proposed CL owns its advantages.