Mechanism And Early Diagnose On Severe Acute Pancreastitis Associated Gut Barrier Dysfunction In Rats

OBJECTIVE By setting up AP model in SD rats,we observe the process ofsevere acute pancreatitis associated gut barrier dysfunction, and investigate the role of secrum TNF-a,IL-6,LPS, ICAM-1 and pathology changes of pancreas and intestinalMETHODS Rats of SAP were induced by retrograde injection of 5% sodiumtaurocholate into the pancreatic duct. forty-eight rats were randomly divided into two groups, namely group SAP and group control. There were three time points for every group (namely at 6h, 12h,24h) Rats of group SAP and group control were killed after observed at 6h, 12h, 24h and the serum levels of TNF-a, IL-6, LPS and the expression of ICAM-1 in the intestinal mucosa were examined respectively at 6,12 and 24 hours after operation for both the SAP group and the control group. were measured respectively. intestinal tissue, pancreas tissue and liver tissue were futher pathologied. At the same time, the bacterial culture of samples from pancreas, liver, and mesentery were made respectively and changes in pathology were observed.RESULTS (1) Compared with control groups, the serum levels of TNF-a,IL-6 LPS, AMY and the expression of ICAM-1 in the intestinal mucosa increased significantly in SAP groups(P<0.01). (2) Compared with control groups, the expression of ICAM-1 in the intestinal mucosa increased significantly in SAP groups (P<0.01), and it significantly difficult when compared with pancreas pathology (P<0.01). (3) The bacterial translocation rate of pancreas and mesentery all were positive in SAP groups when compared with control groups. (4) there was positive correlation between...