| Objective:The study was designed to investigate the intestinal mucosa barrier function changes in rat with severe acute pancreatitis (SAP),and the intervention effect of edaravone injection and its mechanisms of protection on intestinal mucosa barrier function injury of SAP with the purpose of providing evidence for preventing and curing intestinal mucosa barrier function injury of SAP in clinical practice.Methods:A total of 60 healthy male Sprauge-Dawley(SD)rats were randomly divided into norm group(Norm),sham-operation group (Sham), SAP group (SAP), edaravone treated group(ED), and each with 18 rats except for norm group(with 6 rats).The latter three groups each had three subgroups at three points:6,12,24 hours.Rats with SAP were prepared with retrograde cholangiopancreatic duct infusion in 5%sodium taurocholate.The dynamic changes of the indexes of intestinal mucosa barrier function injury and pancreas injury were observed at various time points in each group,including the levels of plasma diamine oxidase(DAO) and serum amylase and malondialdehyde (MDA), superoxide dismutase (SOD), intercellular adhesion molecule-1(ICAM-1) in gut. Pathologic changes in pancreas and gut tissues were observed. Statistical analysis was done by SPSS 13.0 statistical software. Frequency distribution of data was examined by the Kolmogorov-Smirnov test.Data according with normal distribution was described with mean±standard deviation (x±s). One-way ANOVA was used to analyse the difference among groups.If the difference was statistically significant, further using SNK method(q test) to compare between groups. The test correlation uses Pearson analysis.Results:1.Changes of The levels of serum amylase and plasma DAO were not significantly different between Norm group and Sham group.The levels of serum amylase and plasma DAO concentration in SAP group were significantly higher at 6h,12h,24h postoperation than Nrom group and Sham group; The levels of serum amylase and plasma DAO concentration in ED group were lower than SAP group,but higher than Nrom group and Sham group;2.Changes of MDA concentration, SOD activity in intestine were not significantly different between Nrom group and Sham group; Compared with Nrom group and Sham group,The concentration of MDA increased in SAP group at 6h,12h,24h postoperation,but the activity of SOD significantly decreased. The concentration of MDA lower in ED group in all times than SAP group,but higher than Nrom group and Sham group; The activity of SOD was higher in ED group in all times than SAP group,but lower than Nrom group and Sham group.3.Changes of ICAM-1 concentration in intestine were not significantly different between Nrom group and Sham group (P>0.05);Compared with Nrom group and Sham group,The concentration of ICAM-1 significantly increased in SAP group at 6h,12h,24h postoperation; The concentration of ICAM-1 lower in ED group in all times than SAP group,but higher than Nrom group and Sham group.4. Pathologic changes of intestinal tissue in SAP rats included villusand lamina loss,exposed,submucosal vascular congestion and so on. Pathologic changes of pancreatic tissue in SAP rats included edema, bleeding,necrosis and inflammatory cells infiltration.No pathologic damage of pancreatic tissue and intestinal tissue were seen in Nrom group and Sham group;The pathologic damage of pancreatic tissue was alleviated evidently in ED group comparied with SAP group; No pathologic damage of intestinal tissue was seen in SAP group and ED group.5.There was positive correlation between the concentration of plasma DAO and MDA,but negtive correlation between the concentration of plasma DAO and the activity of SOD; There was positive correlation between the concentration of plasma DAO and ICAM-1.Conclusion:The function of intestinal mucosal barrier is damaged in the early phases of SAP.Edaravone can protect the damages of intestinal mucosa barrier function in SAP rats,probably through reducing free radicals,cytokines and the ICAM-1 expressing the endothelia cells. |
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