Effect Of Curcumin On Cholesterol Cumulation And Its Relationship With SREBP-1 Activity In VSMCs

AIM: to investigate the effect of curcumin on cholesterol cumulation and its relationship with SREBP-1 activity in vascular smooth muscle cells.METHODS: first, vascular smooth muscle cells (VSMCs) from rat aorta were treated with curcumin of different density (0, 6, 12.5, 25μmol/L) for 24 hours or were treated with 12.5μmol/L curcumin for different time (0, 3, 6, 12, 24, 48h) . the level of the total cholesterol was detected by high performance liquid chromatography ,and the expression of Caveolin-1 was detected by Western Blot.Second, VSMCs -derived cholesterol-road cells were treated with curcumin of different density (0, 12.5, 25, 50μmol/L ) for 24 hours or were treated with 25μmol/L curcumin for different time (0, 6, 12, 24, 48h) , the level of total,free and esterified cholesterol was detected by high performance liquid chromatography, and the expression of Caveolin-1 and SREBP-1 active fragment was detected by Western Blot.At last,curcumin of 25μmol/L effects on VSMCs -derived cholesterol-road cells with the intervention of SREBP-1 suppressive agent ALLN, oil red O dyeing detects lipid droplet in cells, high performance liquid chromatography detects the level of total,free and esterified cholesterol, and Western Blot detects the expression of SREBP-1 active fragment and Caveolin-1.RESULTS: the total cholesterol level and Caveolin-1 expression were also down-regulated when VSMCs were treated with curcumin of different density((0,12.5, 25, 50μmol/L) for 12 hours,and the total cholesterol level and Caveolin-1 expression were also first down-regulated then up-regulated when VSMCs were treated with curcumin of 12.5μmol/L for different time (0, 3, 6, 12, 24, 48h) . the level of total,free...