Study On The Biocompatibility Of CaO-SiO₂-based Biomaterials For Different Cells

In recent years, a variety of bioactive inorganic materials have been studied as bone substitutes, and the results showed that the CaO-SiO₂-based materials are bioactive, degradable and biocompatible. However none of them are ideal. Therefore, it is important to fabricate new bioactive materials with excellent bioactivity, degradability and mechanical properties for human bone prosthesis. In this dissertation, several novel CaO-SiO₂-based materials including calcium silicate (CaSiO₃), dicalcium silicate (Ca₂SiO₄), tricalcium silicate (Ca₃SiO₅), akermanite (Ca₂MgSi₂O₇), bredigite (Ca₇MgSi₄O₁₆), hardystonite (Ca₂ZnSi₂O₇), magnesium silicate (MgSiO₃) were evaluated their biocompatibility via two types cells such as fibroblasts L-929 and osteoblasts compared with traditional clinical bone tissue biomaterial-calcium phosphate (TCP).In the same condition, the powder was extracted in culture medium RPMI 1640 without serum and the dissolution was dissolved by the same medium to be different concentration. Fibroblasts and osteoblasts were treated with such extract liquid for 1, 3, 6 days, then measured by MTT analysis for cell proliferation. The ion concentration in original extract liquid was measured via ICP.The results showed that these novel CaO-SiO₂ based biomaterials have good properties in biocompatibility, which could increase L-929 and osteoblasts proliferation. And these effects were dose dependence. The different type of cell has different sensitive to ion changing in environment. So L-929 and osteoblasts showed the different proliferation changes in the same concentration extract liquid.The hit of skin tissue wound healing in clinic is how to quicken would healing resulted from injured, burn and canker and how to decrease the abnormal scar formation. Bioactive inorganic materials-Bioglass 45S5? has been proved to have significant effect in hard tissue, bone tissue, wound healing. We wondered whether 45S5 could have the same significant effect on the soft tissue, skin tissue wound healing.In this dissertation, fibroblasts L-929 were treated with bioglass 45S5 dissolution in order to exam the effect of bioglass on the proliferation and migration of fibroblast. The proliferation was analyzed by MTT and the migration was measured by migration distance. The result showed that the low concentration of 45S5 at 0.625 mg·mL^-1 and...