Analysis Of Different Mutation In Regulatory Sequence Of PRL Gene During The Formation Of E₂-induced Prolactinoma In Eutopic And Ectopic Pituitary Of The Rats

The pituitary prolactin-producing tumor (prolactinoma) is one of the fairly common lesions in endocrine system. Most patients with pituitary prolactinoma present with amenorrhea and galactorrhea. Other clinical manifestations, such as visual disturbances, headache, cranial nerve injury and endocrine dysfunction, may caused by oppression of macroprolactinoma on the anterior pituitary or the hypothalamus and hyperprolactinemia. However, so far the pathogenesis of prolactinoma is not very clear. In order to elucidate the mechanisms of pituitary prolactin-producing tumors formation, three parts in the studies were included in this paper: 1. Demonstrating the possibility that E2(17 beta-estradiol)-induced pituitary prolactinoma in SD rats may originate from the pituitary level, except the influences of hypothalamic factors. Male SD rats (30 days old) were transplanted with an extra pituitary under their renal capsules and treated with implantation (s.c.) of an empty or E2 -laden silastic capsule. 2. testing the expression of PRL mRNA in pituitary glands by Reverse transcriptase-polymerase chain reaction (RT-PCR). 3. To analyse the mutation in regulatory sequence of PRL gene using DNA sequencing.The results indicated that: 1. After treated with E2 for 120 days, both the eutopicand ectopic pituitaries were three times more heavier than those from control group(P<0.01) , and the body weight of rats decreased significantly. 2. The PRL mRNAexpression in the prolactinoma of the eutopic and ectopic pituitaries treated with E2...