Probiotic Bacteria Analysis Of 5 Medicinal Probiotic Products

The species and the number of recovered are crucial for probiotic products. Microbial analyses of probiotic oversea have demonstrated that identity and the number of recovered species do not always correspond to the information stated on the product label. However, there was no related research this in our country. This thesis aimed to analyze the numbers and species of five medicinal probiotic products in our country. We investigated the numbers of viable microorganisms of five medicinal probiotic products, using a representative sample sizes, thereby the numbers of viable microorganisms was reflected statistically to the expiry date. And ten strains were identified from products using phenotypic analysis and 16S rDNA sequencing analysis. At the same time, PCR-DGGE analysis was used to identify probiotic microorganisms in products and it appears that PCR-DGGE is a reliable, fast, and reproducible culture-independent method for quality control of probiotic products.Methods and results: (1) appropriate selective media was acquired through selection for enumeration of probiotic strains in five medicinal probiotic products. The enumeration results showed that viable cells ranged from 10⁵ to 10¹⁰CFU, from10⁵ to 10⁸CFU and from 10⁶ to 10⁸CFU respectively in three species of productâ… . Viable cells ranged from 10⁸ to 10⁹CFU in productâ…¡. Viable cells ranged from 10⁸ to 10⁹CFU in productâ…¢. Viable cells ranged from 10⁷ to 10⁸ CFU and from 10⁸ to 10¹⁰ CFU respectively in two species of productâ…£. Viable cells were 10⁷ CFU, 10⁶CFU and 10⁶ CFU respectively in three species of productâ…¤. (2) Isolated and purified strains from probiotic products. Genome DNA was extracted and 16S rDNA gene was amplified from strains respectively. The amplification was purified and sequenced, then compared the sequences we obtained with the sequences in GenBank database in order to identify the strains. And unrooted trees were established. The accession numbers in GenBank of the sequences of 16S rDNA of the strains were followed by PFK1(DQ295034), PFK2(DQ295035), PFK3(DQ295036), LZCL(DQ295040), ZCS1(DQ295037), MMA1(DQ295041), MMA2(DQ295038), JSQ1(DQ295042), JSQ2(DQ295039), JSQ3(DQ295043). The Blast and phylogenetic tree analysis showed that...