Application Of Identification Of Atypical Bacteria With16s RRNA Gene Sequence Analysis

Objective: Established an approach for babacteria identification with16S rRNA gene sequence analysis and applied to routine bacteriaidentification in clinical microorganism department initially, evaluated itsability for routine bacteria identification.Methods: Selected bacteria inaccurate identification by clinicalmicroorganism department, setting primers targeting16S rRNA gene atconserved region, ampilying the certain fragment, then comparating thesesequences with foregone known sequences in the database. When thedetermined sequence yielded a similarity score of≥99%with a referencesequence of a classified species, the unknown isolate was assigned to thisspecies; when the score was <99%and≥95%, the unknown isolate wasassigned to the corresponding genus; and when the score was <95%, theunknown isolate was assigned to a family.Results:11of13strains（84.6%） successed being identified to specieswith similarity>99%, included Enterobacter cloacae、Enterobacter cowanii、Staphylococcus epidermidis、Staphylococcus aureus、Raoultella terrigena、 Acinetobacter baumanii、 Streptococcus lutetuensis、 Enterococcuscasseliflavus、 Streptococcus parasanguinis、 Uruburuella suis、campylobacter jejuni, but Acinetobacter lwoffii only could be identified togenus with similarity95%.Conclusion: The16S rRNA gene sequence analysis could identifyatypical bacteria quickly and accurately,it could be an supplementary testfor routine bacteria identification.