Experimental Study Of Peroxisome Proliferators-activated Receptor-α, γ In Acute Lung Injury Rats Induced By Lipopolysaccharide

ObjectiveAcute lung injury (ALI) is a kind of clinic syndrome with the character of an aggressive inflammatory reaction and permeable pulmonary edema driven by significant permeability of pulmonary microvascular, and its severe phase is acute respiratory distress syndrome (ARDS). The mechanism of ALI/ARDS remains not clear. Patients with ALI/ARDS suffer a higher mortality rate, although much effort has been invested in the development of therapeutic strategies for ALI/ARDS. Peroxisome proliferator-activated receptors(PPARs)are a family of ligand-activated nuclear hormone receptors belonging to the steroid receptor superfamily. To date, three different PPAR subtypes have been identified: PPARα, PPARβ(also referred to as PPARδor NUC-1)and PPARγ. Recently, PPARαand PPARγhave been suggested to be important inflammatory modulators. However, the expression of PPARs and the potential anti-inflammatory properties of PPAR agonists on ALI/ARDS have not been investigated. Therefore, in this study, we investigated①the expression of the mRNA and the protein of PPARαand PPARγin rats lung induced by lipopolysaccharide(LPS),②the effects of wy14643 and Troglitazone, PPARαand PPARγagonists, on the expression of PPARαand PPARγin ALI rats lung and③the mechanism of the agonists of PPARαand PPARγin protecting lung tissues against acute lung injury related to LPS. The aim of this research is to provid theoretical proof of ALI/ARDS therapy.MethodsThe model of ALI rats was established with LPS (5mg/kg) originated from E.coli. 40 male Wistar rats were randomly divided into five groups: NS(normal saline)control group...