Effects Of Insulin-Like Growth Factor On The Proliferation And Differentiation Of Cultured Neural Stem Cells Of Embryonic Mice In Vitro

Objective:The discovery of neural stem cells(NSCs) make it possible for the reparation of central nervous system(CNS). Now, differentiation of the NSCs is one of the most interesting fields in neuroscience research. It also is one of the problems for the clinical application of the NSCs. This experiment is to explore the effects of IGF-1 on the proliferation and differentiation of cultured stem cells of embryonic mice in vitro.Mothods:Cerebral cortex was dissected out from E14 embryonic mice and made into single cell suspension,, which cultured by the technique of serum free cultivation combined with growth factors (EGF and bFGF). The characteristics of NSCs were dectected in different growing phases. NSCs were subcultured continuously. After 3 passages, adding variant concentrations of IGF-1 to investigate the proliferation and differentiation of NSCs. The method of MTT was used to identify the proliferation of stem cells. And immunofluorescence was performed to identify the differentiation result of NSCs with different additional components. All the data were were analyzed by statistics.Results:NSCs isolated from the E14 embryonic mice's cerebral cortex continuously proliferated and formed neurospheres. The results of immunofluorescence showed: either the primary or the passaged neurospheres were Nestin positive. And the differentiated cells were GFAP...