Establishment Of A Novel Method For Preparing Embryoid Bodies And Experimental Studies On New Blood Vessel Formation From In Vitro Differentiation Of Mouse Embryonic Stem Cells

Background and objectiveMurine embryonic stem (ES) cells are pluripotent cell lines of embryonic origin, first isolated from the inner cell mass (ICM) of the preimplantation blastocyst. When cultured without feeder cells such as mouse embryonic fibroblast (MEF) or in the absence of anti-differentiation factors as leukemia inhibitory factor (LIF), ES cells can spontaneously develop into 3-dimensional, multicellular aggregates called embryoid bodies(EBs), which contains structures of endoderm, mesoderm and ectoderm. As an ideal model for in vitro ES cells differentiation, EBs recapitulate many aspects of the lineage specific differentiation programs and temporal and spatial gene expression patterns of early embryogenesis. Therefore, the EBs system is widely used in investigating early embryonic developmental events such as mutual induction of germinal layers, organ cavitation and so on. Furthermore, in the progress of ES cells'induction to tissue specific differentiation, EBs are usually needed to switch on differentiation programs. So preparation of EBs has been an indispensable tool for investigation in stem cell fields. By now,...