The Effect Of Embryoid Body State On The Neural Induction By Mouse Embryonic Stem Cells

Embryonic stem cells can in theory produce all cell types of a living organism while renewing themselves with a stable genetic background. These unique features make embryonic stem cells a favorable tool for biomedical researches as well as a potential source for therapeutic application. In vitro, mouse embryonic stem cells can not only spontaneously differentiate into ectoderm, mesoderm and endoderm, but also directly differentiate in the environment which contains special cytokine and chemical molecules. After decades of development, the formation of neural cell lineages from embryonic stem cells has been well established. At present, the majority of protocols for nerve induction through embryoid bodies(EBs) formation are based on the suspension method. However, the formation of EBs with suspension method has defects in random EBs size and number which will affect the efficiency of neural induction and eventually have poor replicabilities. In this study, we carefully controlled the culture conditions for EBs formation, and discussed the factors that influence the neural induction efficiency.