The Studies On The Protective Effect Of Ischemic Postconditioning On Spinal Cord Ischemia/reperfusion Injury In Rabbits

Objective To evaluate the protective effect of ischemic postconditioning on ischemia/reperfusion injury of the spinal cord in rabbits, and to investigate the optimal time of postconditioning. Methods Thirty male New Zealand rabbits were randomly divided into 5 groups with 6 rabbits each: sham group, control group, postischemic treatment for 15s group (PA), postischemic treatment for 30s group (PB) and postischemic treatment for 60s group (PC). Rabbits in control group underwent occlusion of the infrarenal abdominal aorta (IRA) for 15 min followed by reperfusion for 48h. In PA, PB and PC groups, rabbits underwent three cycles of ischemia/reperfusion with the duration of 15s/15s, 30s/30s and 60s/60s respectively just before reperfusion after 15 min occlusion of IRA. Motor function score of the hind limbs was assessed and the number of normal motor neurons at anterior horn of the spinal cord was recorded at 48 h after the last reperfusion. Results Motor function score of the hind limbs in PB group [3.5(2~4)] was significantly higher than that in control group [2(1-3)]...