Electro Myocardial Ischemia And Reperfusion Injury Of Oxygen Free Radicals Effects And Mechanisms

To ascertain that electroacupuncture had a protective role on myocardial ischemia reperfusion injuries by weakening injury reaction induced by oxygen free radicals, explore the possible mechanism, provide us with more proof of correlation between Neiguan point and heart and the foundation of clinical popularize .MethodsForty rabbits were randomly divided into four groups:For shamoperation group, the chest was opened but the coronary artery wasn' t ligasted and the acupuncture wasn' t performed; was carried out by ligation of left anterior descending branch of coronary artery without acupuncture; EA ( electroacupuncture ) -Neiguan group was treated as model group,while the acupuncture was performed 30 minutes after ligation of coronary artery and lasted 30 minutes,was punctured 0. 5 cm lateral to the Neiguan. The changes of SOD, MDA and the activity of cAMP in organization (serum) were observed 60 minutes after reperfusion.Resultsl.The level of cAMP,MDA were increased significantly and the activity of SOD was reduced greatly in model group compared with shamoperation group ( P<0. 01 ) .2. The level of cAMP, MDA were reducedsignificantly and the activity of SOD was increased greatly in EA-Neiguan group compared with model group (P<0. 05 j?P<0. 01) .3. The level of cAMP,MDA were reduced significantly and the activity of SOD was increased greatly in EA-Neiguan group compared with EA-non-point group ( P<0. 05 ) .4.The level of serum SOD and MDA had relationship with cAMP 60 minutes after reperfusion ( P<0. 05 ) . ConclusionThe result of this research indicated peroxidation injury reaction was one of myocardial ischemia reperfusion injury mechanisms; EA on Neiguan point had a protective role of weakening peroxidation injury reaction induced by free radicals on ischemia-reperfusion-induced myocardial injuries, its mechanism might be relevant to decrease the extremely high amount of cAMP by EA, which induced to strengthen the vitality of SOD and to weaken peroxidation injury reaction. Neiguan point exerted protective effects mentioned above, whereas no-point didn' t exert obvious effects, so there was specificity of acupoint, which testified the correlation between Neiguan point and heart.