The Development Of Thymocytes In Conditional MPTA1/CD226 Transgenic Mice

Objective: To establish the conditional tetracycline-regulated transgenic mice overexpressing mouse platelet and T cell activation antigen 1 (mPTA1) , and study the development of thymocytes in the transgenic mice.Method: The mPTAl expression vector was constructed by recombination of pcDNA3-mPTA1 and pBI-5, and the transgenic mouse lines were generated by microinjection using standard techniques. Positive animals were selected based on analysis of DNA sequence from mouse tails by PCR. The controlled regulation of the integrated expression unit was tested by transfection of the primary mouse ear fibroblasts obtained from DNA positive founders with the rtTA plasmid pUHD-17.1. Founder animals that showed DOX dependent expression of luciferase were selected and interbred with C57BL/6 mice to establish F1 of P_tetmPTA1 transgenic mice with defined genetic background.Double-transgenic animals containing the luciferase/mPTA1 transcription and expression tTA in thymocytes were obtained by crossing individuals of...