Study Of Tissue Engineering Nerve Graft With Biological Activity In The Repair Of Peripheral Nerve Gaps

The reparation of peripheral nerve injury is always one of the hotspots in pathology, traumatology, rehabilitation medicine and molecular biology. The treatment of nerve injury was improved with the development of microsurgery. The autoallergic nerve graft could not satisfy the need to repair the long-defect nerve injury because of the limited resource and scarification of sensory function at donor sites. It is necessary to find a kind of nerve substitute.In this study, the heterogenic nerve from pigs was used to get accellular nerve scafford and combined with the neurotrophic factors from schwann cells. The grafts were implanted into the rat's sciatic nerve defects to study the effect of this method. The experiment could be divided to 4 parts:1. The in vitro culture of Schwann cells of adult SD rats: the Schwann cells culture condition was improved to acquire enough Schwann cells for nerve regeneration. The Schwann cells were taken from the SD rats' sciatic nerve. The cells was indentified and purified to over 97%.2. The extraction of Schwann cell neurotrophic factors: the Schwann cells were cultured, collected, crushed and centrifuged to acquire the supernate liquid. The supernatant was treated with dialysis, freeze-dry, and gel filtration chromatography (GFC). Two eluting peaks were got after the Sephadex G-100 GFC and ultrafiltrated. The MTT method and flow cutometry were used to examine the influence of two eluting peaks to the Schwann cells activity and growth cycles.3. Production of the acellular nerve: the pig intercostals nerve was treated with hypotension, -80 ℃ repeatedly frost thawing and NaOH spallation to produce the acellular xenograft with good biocompatibility. The acellular nerve...