Preparation And Identification Of Monoclonal Antibodies And Anti-Idiotype Antibodies Against Aeromonas Caviae

Aeromonas caviae is a pathogenic bacteria that can infect various farmed and wild fish, shrimp, frog, et al and cause great economic loss in aquaculture.It can also infect people by food chain.For adapting to the development of fish culture,and for convenient detecting,curing and preventing the infection of this bacteria, We developed and identified the monoclonal antibodies (mAbs) and anti-idiotype mAbs (AId mAbs) against Aeromonas caviae. There were a series of experiments and the methods and results were as follows:1. Hybridoma technology was used to produce mAbs against Aeromonas caviae, by fusing of mouse Sp2/0 myeloma cells and spleen cells of BALB/c mouse immunized by inactivated Aeromonas caviae.The positive clones which produced mAbs against Aeromonas caviae were screened by enzyme linked immunosorbent assay (ELISA).After cloning,seven strains of hybridoma named respectively 0E10, 1A2, 1C4, 1F1 , 1F10, 1H8 and 2A1 stably secreting specific mAbs against...