The Expression Of HLA-G In Placentas From Women With Preeclampsia And Normal Pregnancy

Preeclampsia (PE) is a pregnancy specific disorder which has a high incidence, approximately 9.4% in China. It is the second cause of maternal mortality and is a major threat to maternal and fetus health. The pathogenesis of this disease has remained unknown. Human leukocyte antigen (HLA-G) is a major histocompatibility complex (MHC) and belongs to nonclassical HLA-1 molecule, it is predominantly expressed on the surface of extravillous cytotrophoblast cells at maternal-fetal interface that associate with maternal-fetal immune tolerance. The maternal-fetal immune tolerance is essential to establishment of successful pregnancy. A few studies showed that the immune factors are critical to the causes of PE. Therefore, it is necessary fto investigate the role and significance of immune tolerance factor HLA-G in the pathogenesis of PE.ObjectiveTo investigate the expression of HLA-G isoforms in placentas from women with severe PE and normal pregnancy and discuss the roles of this molecular in the pathogenesis of PE.Methods1. HLA-G mRNA level in fifteen placentas from severe PE (study group) and fifteen from gestational normal pregnancies (control group) were quantified using real time RT-PCR (TaqMan probe method), respectively. β-actin was used as endogenous control.