Effect Of Double Fragments Coexpression ShRNA Targeting Heparanase Gene On Biological Behavior Of Ovarian Cancer Cells SKOV3

The death rate of ovarian cancer is the highest among tumors of female reproductive system, it is prone to metastasis and diffusion. Traditionary therapeutic methods include the combined therapy of operation, radiotherapy, chemotherapy, but ovarian cancer is still quite hard to cure, five-year survival rate ranges from 20 to 30 percent. So it has become a focus problem to discover new therapeutic methods and inhibit cancer metastasis and diffusion effectively home and abroad. The mechanisms of reduplication, adherency, invasion and metastasis of tumor cells are the interactive cascade reaction results of multiple gene, multiple steps and multiple stages. Scientists have done many deeply and extensive researches in this domain and got some achievements, but have not find an effectively inhibition method.Heparanase is now recognized as a substance that has close relation with tumor metastasis, people can effectively inhibit tumor metastasis by blocking it. RNAi technology is now recognized as the most effective target gene silencing technology, this experiment analyses the gene silence effect of...