Primary Explore For The Effect Of Recombinant Human BMP4 Mature Peptide On The Bone Marrow Hematopoiesis Of Mouse With Irradiation Injury

Bone Morphogenetic Proteins ( BMPs ) are a kind of secretive proteins and have many important physiological functions. BMP2 and BMP4 are two important members of BMP family, they have many similar structures and physiological functions. BMP2 can induce the formation of osteoblasts and so it is used as one of important factors in the bone tissue engineering.As a hematopoietic growth factor, BMP4 has an important role in the development of the hematopoietic system.And the more important is that BMP4 can promote the proliferation of the hematogenous stem cells.In genetic engineering, there are two kinds of expression system for preparing recombinant human BMP (rhBMP), eukaryotes and prokaryotes. Eukaryotic expression system can give products with good activity. But it has common problems such as low production, difficult purification and high costs that make the products too costly to be widely used in clinic. And these problems are not easily to solve. Expressing rhBMP mature peptide (rhBMPm) in Eschericha coli can solve such problems. So we choose the expression system of Eschericha coli to produce rhBMP-2m or rhBMP-4m in large scale. At present there are many report for the prosess of producing BMP2 in large scale, our aim...