Gene Expression Profile Of Malignant Melanoma

Malignant melanoma (MM) is commonly formed with rapid proliferating melanocytes. MM is a highly malignant skin carcinoma with a high mortality rate, poor prognosis, and easy metastasis. Since the molecular pathogenesis of MM remains unknown, by using new approches to investigate the pathogenesis of MM will be very helpful for developing a more efficient prevention and treatment strategy for MM in clinic.Objective: To identify the gene expressing profile in malignantmelanoma(MM), confirm their expression in MM at protein level by immunohistochemistry, and analyze the relation between gene expression pattern and MM pathogenesis.Methods:(1) RNA was extracted from four MM tissues and four melanocytic nevus (MN) tissues. Agilent Human 1A OligoDNA chips were employed to the exploration of the difference in gene expression profile in MM compared to MN. (2) Immunohistochemistry was used to further confirm the results of the gene expression of Vascular Endothelial Growth Factor (VEGF).