The Separation Of Heat Shock Protein70-peptide Complexes From Esophageal Tumor Cell Line And The Study Of Its Anti-tumor Activity

BACKGROUNDEsophageal tumor is one of the most popular malignant rumors. In spite of decade years of effort on the surgical treatment of esophageal tumor, there is still exigent need of new treatments to improve the survival rate after the treatment with high-dose chemotherapy and surgical therapy. Vaccine transferring can elicit the immune activity of patients and induce the anti-tumor response to overcome tumor.HSP70, members of HSP family, was found to be most abundant in cellular cytoplasm as conservative protein and was highly expressed in heat shock. It was reported that HSP70 play important roles in tumorigenesis, such as the genesis of esophageal tumor. The HSP70-peptide complexes separated from several tumors such as hepatoma have been proved to be able to elicit anti-tumor immune response in vitro and in vivo. However, the same results have not been reported as to esophageal tumor.HSP70-peptide complexes pulsed with DC or linked with tumor antigen were the popular vaccine type in HSP vaccine. In different tumors, the anti-tumor effect was different according to the application mode of vaccines. Identification of the most effective vaccine of HSP will be the crucial progress to improve the effect of tumor vaccine.