The Mechanisms Of Hypoxic Ischemic Brain Damage And Influencing Factors

Perinatal hypoxic-ischemic brain damage (HIBD) remains a major cause of acute mortality and chronic neurological disability in infants. The mechanisms of brain injury are complicated and not completely understood. No therapeutic strategy has been proven clinically effective. The extent of hypoxic-ischemic injury may depends on the severity and duration of the insults. Other factors, such as gender, environment temperature and brain maturation may also have some effect on brain injury. The purpose of this study was to investigate the effects of cerebral maturation, gender, circumstantial temperature on brain damage after HI; the age and gender related cell death mechanisms; the age related changes of glutamic acid decarboxylase-67 expression; the effects of X-linked inhibitor of apoptosis(XIAP) overexpressing on apoptosis related protein expression and oxidative stress in a mice model of HI brain injury.Materials and MethodsC57BL/6 mice on postnatal day 5 (P5), P7, P9, P21 and P60 or P9 XIAP overexpressing mice were subjected to left common carotid artery ligation plus hypoxia. Animals were sacrificed at certain time points after hypoxia-ischemia(HI). The brains were collected for immunohistochemistry, immunofluroscence, Western blotting, enzyme activity measurement and brain injury assessment. The sex of the immature mice (P5, P9) was identified by PCR.