| BackgroundThe epidermal cell (keratinocyte) culture may be used for exploring the epidermal biology character and the pathogensis of the skin diseases, also for skin pharmacology; especially it may have insight for burning and plastic surgery. The human epidermal cell culture in vitro has been carried out for a long time, more than 100 years; however, a problem about how to culture a great amount of the epidermal cells in vitro could not be resolved, until the technique, ie., the lethally irradiated NIH-3T3 fibroblasts used for as a culture feeder, was improved by Rheinwald and Green. With the rapid development of molecular biology and tissue engineering and further improvement of the experimental conditions, the keratinocyte culture technique has been improved uninterruptedly in theory and practice.Various conditions for keratinocyte culture in vitro have been reported in home and abroad. However, it has not yet been reported about further comparative studies on detailed digesting keratinocyte conditions, which affected the cell biological characteristics, such as the clone formation rate, the confluent time and etc. It was explored that the optimal conditions for bioengineering of human epidermal to establish optimal stable culture conditions, including the problems of feeder layer, cultured media contents, especially about the digesting keratinocyte conditions in this experiment. Besides, the epidermal epidermal cells were filtered by using different nylon filters with four kinds of "Mus" in order to obtain more numerous numbers of deep layer epidermal cells and to obtain the ratio of deep layer and middle/superficial epidermal cells. This experiment would provide theoretical and experimental basement for epidermal biology engineering.
|
PDF Full Text Request