Experimental Study Of Composite Of Silk Protein Scaffolds And Osteoblasts Induced From Bone Marrow Stromal Cells To Repair Bone Defects

Objective: To investigate the possibility of using composite of silk protein scaffolds and osteoblasts induced from bone marrow stromal cells(BMSCs) in repairing rabbit bony defects.Methods: BMSCs were obtained from long bone of Zelanian rabbits aged 4-8 weeks,the cells were isolated by gradient centrifugation and purified by anchoring culture.The acquired cells were propagated and cultured in vitro.The second passage cells were induced to differentiate into osteoblasts by means of an induction differentiation medium.Alkaline phosphatase(ALP) activity and the ability to form mineralized nodules were examined.The third passaged osteoblasts induced from bone marrow stromal cells(BMSCs) in an appropriate density(2×107cell/ml) were seeded in silk protein scaffolds and formed into a complex. The complex(A group) and silk protein scaffolds (B group) were transplanted into the radius bone defect of rabbits. The radius bone defect of rabbits which were undone were C group.The rabbits were sacrificed 4,8,12 weeks after implantation and the results were evaluated by roentgenograms,Lanes Radiographic Scoring,Histological Nillsons Scoring.Results: Colonies of stromal stem cells were formed in the primaryculture and contacted one another at 12 days. In the passage culture,cells became bigger than those in the primary culture and could be...