BFGF Transfected Bone Marrow Stromal Cells And Scaffold Materials For Animal Experiment

Objective: The mechanism of tissue engineering bone, observed by bFGF genelentiviral vector transfection ovine BMSCs without transfection of BMSCs tissueengineering bone, allogenic bone scaffold material,-TCP material group four material torepair the bone defect of sheep iliac limit.Methods: The experiment was divided into4groups according to the materials, group Awill be bFGF gene recombinant lentiviral vector, transfection of sheep BMSCs afterallogeneic bone scaffold materials (transfection group), group B without transfection ofBMSCs loading allogeneic bone material frame (no transfection group), group C withallograft bone scaffold material, group D β-TCP materials,4groups of sheep model ofbilateral iliac bone graft material limit defect, grouped by4,8,12weeks at3time points,3rats in each group, a total of9animal images, respectively, and histological observationat three time points, to observe the12weekend in scanning electron microscopeResults: Imaging results showed as follows:4weeks, there was no significant difference(P>0.05);②8weekend transfection group were significantly higher than those of othergroups of materials (P<0.05);③without transfection group was better than that of simplescaffold group and β-TCP group; there was no significant difference of allogeneic bonesupport material and β-TCP material. The histological results showed: HE staining,osteogenic group A than in group B than group C,D. Scanning electron microscopyshowed that: cell adhesion of A group>B group>C, group D.Conclusion: Proved after gene transfection of tissue engineering bone in repairing bonedefects without limit is better than that of transfected cells scaffold materials and simplescaffold material and β-TCP material; without the cells transfected bone scaffold materialwas superior to pure allogeneic bone scaffold material and β-TCP material group; simple bone scaffold material and β-TCP material without significant differences between.