The Induction Of DCs From Multiple Myeloma Patients In Vitro And Study Of Its Biological Characteristics

Multiple myeloma is a malignant disease involving plasma cells, usually accompanied by the production of an Ig paraprotein of a defined idiotype. Until recently, it has been clear that optional management of the disease involved reatively low-dose chemotherapy (eg. Melphalan and prednisone), resulting in a median survival about approximately 3 years. It has become clear that more intensive chemo-therapy.followed by autotogous stem cell transplantation,will prolong median survival of the patient, but who is still not to be cured completely.Dentritic cells are known to be the most potent professional APCs. Immature DCs had specialized antigen uptake and processing machineries, whereas mature DCs had an extraordinary capacity to present antigens and stimulate native T cells. Functionally, mature DCs were 100 times more potent than macrophages in activating native T cells in vitro. MDDCs (monocyte -derived dentritic cells) from MM patients have been reported to have obvious effect have obvious effect on resisting tumor activities. MDDC pulsed with idiotype administered administered to individual patients induced a T lymphocyte proliferative response and an anti-idiotype antibody response. Because MM patients are highly tendentions to the MRD (minimal residual disease) state after autologous stem cells transplantation, DC-based vaccination strategies, or Idspecific CTL infusion after transplantation may prolong disease-free survival and postpone he interval of relapse.Objective The present study aimed to obtain DCs from MM and study its biological function in vitro, so that providing experiment reliance based at DCs for MM immune remedy.