Experimental Study On The Contribution Of Bone Marrow Mesenchymal Stem Cells To Renal Repair Of Ischemia-Reperfusion Injury

Objectives:Acute renal failure(ARF) is a serious clinic syndrome, currently no drastic management is available to improve the renal fuction other than dialysis. The acute tubular necrosis(ATN), caused by ischemia-reperfusion injury is one of the most common reason of ARF. The pathophysiologic abnormalities of ATN include intrarenal vasoconstriction, a decreased glomerular filtration pressure, tubular obstruction, transtubular back-leakage of the filtrate, and interstitial inflammation. This experimental work is aimed to explore the possibility of mesenchymal stem cells(MSCs) differentiating into cells with renal tubular cell phenotype in vitro, and to identified the contribution of MSCs to the renal repair of ischemia-reperfusion in vivo.Methods: 1. In Vitro Experiment: after isolation and purification, Rat MSCs were induced by 5μM all-trans retinoic acid (ATRA) and ischemia-reperfusion injured kidney tissue homogenate separated by a semipermeable membrane.