Establishment Of Pancreaticoduodenal Transplantation Model In Rats With Duodenostomy And Experimental Study On The Role Of Costimulatory Signal In Acute Pancreas Allograft Rejection

Part I Establishment of Pancreas Transplantation Model in Rats with duodenostomyObjective To establish a physiologic rat model of pancreaticoduodenal transplantation for basic research.Methods Diabetes was induced by a single intravenous injection of streptozocin (STZ) at dose of 50mg/kg of body weight. The nonfasting blood glucose and urine glucose of the rats were assayed before the inducement of DM and measured on alternate day after the injection. Only rats with nonfasting blood glucose exceeding 16. 8mmol/L and the strong positive reaction of the urine glucose were selected as recipients. The whole pancreas duodenal transplantation model was adopted. The aortic segment of graft , including the celiac and superior mesenteric divergences, was end-to-side anastomosed with recipient abdominal aorta. While the donor portal was end-to-side anastomosed with the branch of recipient superior mesenteric vein. And the graft duodenum was ligated in approaching end and was made a stoma in distant end. While the...