Effect Of Ischemic Preconditioning On Nuclear Factor-κB Activation After Orthotopic Liver Transplantation In Rats

Objective Ischemic preconditioning (IP) has been considered as an effective strategy for protecting the graft from cold preservation injury after liver transplantation, however, The exact mechanism of IP is unclear, In this research , we study the effect of ischemic preconditioning on the activity of nuclear factor-κB (NF-κB) during reperfusion early after liver transplantation in rats and the significance of the effect on the liver graft.Methods The model of orthotopic liver transplantation in the SD rats was established. The donor liver was stored 2 hours in Ringers solution at 4℃ pre-implantation. All rats were randomly divided into: Sham group, Control group and IP group. IP was done by clamp of the portal vein and hepatic artery of the donor liver for 10 minutes followed by reperfusion for 10 minutes before harvesting. The activity of NF-κB and expression of tumor necrosis factor-α(TNF-α) and intercellular adhesionmolecule-1 (ICAM-1 ) within 6 h after reperfusion were examined. Serum alarming aminotransferase (ALT, LDH) was also evaluatedResult Recipients with IP significantly improved their liver...