Protective Effect Of Total Flavones Of Rhododendra On Myocardial Ischemic Injury And Its Mechanism

Total flavone of Rhododendron (TFR) is the effective part, which was extracted from the flower of Rhododendron. Recently many kinds of flavons have been found to have protective effects on myocardial ischemia injury. The main component of TFR are quercetin, hyperin and matteucinol, quercetin and hyperin have apparente protective effects on myocardial ischemia injury. But till now ,there is no report about the effect of TFR on myocardial ischemia injury. In this paper, the protective effect of TFR on myocardial ischemia injury and its mechanism were studied. Results were showed as followed:1. On the model of myocardial ischemia mice induced by Iso ,TFR 25, 50 mg ? kg^-1 iv markedly inhibited Iso-induced the raise of the ST segment of ECG and the increase of MDA content in mice serum, TFR 50 mg?kg^-1 iv inhibited the increases of serum LDH activity and myocardial MDA content in mice;2. On the model of ischemia-reperfusion rat heart in vitro, TFR 20, 40, 80 mg ? L^-1 significantly inhibited the reductions of coronary flow and myocardial SOD activity induced by myocardial ischemia and reperfusion in isolated rat heart, TFR 80 mg?L^-1 inhibited the increase of MDA content and the reduction of the SOD activity and NOS activity in rat myocardium, TFR 40 mg ? L^-1 inhibite the reduction of NO content in rat myocardium.3. On the myocardial infarction model by occluding the anterior descending of the left artery (LAD) in rats, TFR100 mg ? kg^-1 obviously reduced the height of ST segment after occluding 30mins and TFR 25, 50, 100 mg ?kg^-1 obviously reduced the height of ST segment after reperfusion 30mins. TFR 50mg ? kg^-1 reduced infarction area .