Characteristics Of Intestinal Absorption And Transport Of Famotidine And Effects On Them By Mucosa Protective Agents

Background: As one of the histamine H₂ receptor antagonist, the oral absorption of famotidine is not complete, its bioavailability is about 37%-50%. There are some reports about the character and mechanism of famotidine absorption, but there is no agreement on this problem commonly. The combinative treatment of famotidine with mucosa protective agents is usually to treat acid-related disease, but there is few report about the interplay effects of this two aspects.Objective: To study the absorption and transport of famotidine in Caco-2 cell model, and its absorptive kinetics in rat intestine and the discrepancies of the segmental intestines, as well as the effects on famotidine absorption by the mucosa protective agents including teprenone and sucralfate.Methods: On the establishment of the Caco-2 cell model, the research is carried out to study â‘ the effect on famotidine absoiption by different incubation time, pH value and drug concentration, â‘¡the transport of famotidine and â‘¢the effects of teprenone and sucralfate on famotidine absorption and transport; On the establishment of the intestine absorption model of rat in vitro and in situ, the research is carried out to study â‘ the discrepancies of the segmental intestines and the absorptive kinetics of famotidine, â‘¡ its effect by teprenone and â‘¢including intragastric administration of teprenone on the the rat experiment. The concentration of famotidine in samples was determined by HPLC method.