The Culture And Identification Of Rat MSC Cell Lines Modified By Osteoprotegerin Gene

Objective: In order to understand the ability of expression and secretion ofeukaryotic plasmid of osteoprotegerin, we transfected mammalian secreted expressive pSecTag2/B-OPG vector into rat MSC and detected its expression in rat MSC cell lines in this study, to prepare for gene engineering therapy of periodontitis.Method: Rat MSC cells were isolated by a density gradient and cultured from adultSD rat. The constructed recombinant plasmid was transfected into rat MSC cell line and OPG expressed in rat MSC cell line was identified by immunohistochemistry and western blot analysis.Result: Rat MSC cells were separated and cultured. Recombinant plasmidpSecTag-opg was successfully transfected into rat MSC cell line by lipofectamine and OPG-expression cell line of rat MSC was selected and confirmed.Conclusion: OPG-expression rat MSC cell line was successfully established and identified.