Study On Pharmacokinetics And Pharmacodynamics Of Compound HuangBo Granule

The purpose of this research is to study Pharmacokinetics and Pharmacodynamics of Compound HuangBo Granule. Berberine of Compound HuangBo Granule was determined by RP-HPLC assay and it can be used for quality assessment; Pharmacokinetics of Compound HuangBo Granule in mice and the Pharmacokinetic parameters were investigated; different in vitro and in vivo experiments were designed to test its various pharmacological effects. These experiments were consisted of three parts as follows:1. Reverse Phase High performance Liquid Chromatography(RP-HPLC) Determination of Berberine in the Compound HuangBo GranuleThe RP-HPLC assay was invested to determine the content of hydrochloric berberine in Compound HuangBo Granule. The chromatogram condition was optimized as follows: Hanbon-C₁₈ column which is 4.6mm × 250mm and 5 u m as a stationary phase, acetonitrile-water(50:50, KH₂PO₄ 3.4g and SDS 1.7g in 1000mL solution) as a mobile phase, eluent rate of 1 mL/min, detection at 346nm, temperature of column is 30℃. Berberine was quantified with external standard method. The experimental result showed Berberine was completed separated form its impurities and a good linear relationship between the peak area and mass concentration of the berberine within the range of 3.0 - 15.0 μ g/ml(r=0.999749). The relative standard deviation(RSD) was 2.04%(n=6). The average recovery was 100.25%. The content of hydrochloric berberine is 0.4385mg/g Compound HuangBo Granule.2. Study on Pharmacokinetics of Compound HuangBo Granule in MicePharmacokinetics of orally administered at a dose of 25 g/kg Compound HuangBo Granule(10mg/kg berberine) were investigated in mice. Serum concentration of the effective component-hydrochloride berberine were measured using HPLC and concentration-time data analyzed with Pharmaceutical Kinetics Software. The results showed that the serum profiles of the drug's effective component—berberine were best described by a two compartment open model, the...