A Study On Subacute Toxicology Of Tinidazole In KM Mouse And The Determination Of The Elimilation By RP-HPLC

Tinidazole is a nitroimidazole compound active in treatment of anaerobic protozoan and bacterial infection. It is considered to be carcinogenicity and maximum residue limit in any animal edible tissue is zero.To detect the residues in plasma and muscle and liver this three tissues, to find out the best tissue for determination. This study is aimed at developing a reverse phase and provide a method for speed determination without danmage and waste by the high performance liquid chromatographic method. And explore the possible toxic reaction and offer trustworthy toxicology evidence in animal food. According to the consisitence data of plasma and muscle and liver tissues by the RP-HPLC in this paper, simulate the tendence of the elimilation in KM mouse and then offer the possible elimilation time in animal food.Divided the 100 KM mouses into 2 parts. The first part was divided into 5 teams, each team was breathed TNZ solution of 100mg/kg weight into stomach in 5 days continuously. After 5d, the teams was put into death in order to get the sample in different times. Then put the plasma samples into centrifugal machine centrifugated under 4℃at 13000r/min for 10mins and got blood serum. Mixxed 0.5ml blood serum and 0.2ml methanol, vortex for 1 min, then put 0.8ml acetonitrile, vortex mixing for 3-5min, put the mixture extractive more than 10min. Then centrifugated the mixture under 4℃ at 10000r/min for 10mins. Transferred the supernatant to a new tube, centrifuged at 10000r/min for 10mins once more, and the supernatant was directly injected into the HPLC system. Muscle and liver tissues was broken and got the supernatant injected into the HPLC system to be determined as same congditions as the plasma.The second part was divided into 4 teams for the subacute toxicology experiment for 30d. The terms were breathed TNZ solution of 0mg/kg,100mg/kg, 500 mg/kg, 1000 mg/kg weight dose into stomach. Collected the samples after killing every team. A part of the plasma samples were determined by automatic enzyme machine. Another part was...