The Experimental Study Of The Repairing Of Cartilage Deffects Using MSCS With PLGA Scaffold

ObjectiveArticular cartilage injury is a common disease in the field of orthopedics, whose incidence caused by aging, articular wear, degeneration, trauma and sports injury is increasing. It has been a very important factor leading to disability and reduced quality of life. The previous methods to repair the articular cartilage defects do not always attain satisfying results. Reconstruction of cartilage defects still remains one of the most difficult problems for joint surgeons. Since 1980's, the sharp development of tissue engineering provides great chances to deal with this difficult challenge. Significant achievements in co-culturing chondrocytes and scaffolds to repair the cartilage defects by using techniques of cartilage tissue engineering have been obtained. In the meantime, the work to seek after suitable seed cells and scaffold materials for cartilage tissue engineering is ongoing strenuously. The goal of our study is to evaluate the effect of treating the cartilage defects with cell- PLGA composites implanted into osteochondral defects on canine models by using techniques of mosaicplasty, induced BMSC-PLGA scaffold composites in the top of the defect and BMSC-PLGA scaffold composites in the bottom of the defect, osteochondral composites were constructed in vivo, looking forward to acquire desirable seed cells and scaffold materials. Materials and Methods 1 Three healthy Beagles, approximately 10-12 months of age were used in this study.