JNK/P38 MAPK Involved In Ginsenoside Rb1 And Rg1 Attenuating Beta-amyloid Peptide 25-35-induced Tau Protein Hyperphosphorylation In Embryo Rat Cortical Neurons

Objective To investigate the effect and the molecular mechanism of aggregated beta-amyloid peptide 25-35 (Aβ_25-35) on the level of tau protein phosphorylation in embryo rat cortical neurons through nNOS-JNK/p38MAPK ways, and to explore the protective effect of gisenoside Rb1 and gisenoside Rgl on Aβ_25-35-induced tau protein hyperphosporylation and their mechamism of activation of JNK/p38MAPK.Method Primary cultures of cortical neurons were prepared from the embryonic day 18±2 Sprague-Dawley rats. And choose appropriate concentration of Aβ_25-35. wesern-blot and immunocytochemical stain were performed to observe the tau protein phosphorylation and the expression of JNK/p38MAPK or nNOS when neurons exprosed to 20μmol/L Aβ_25-35 in the prescene or...