Expression And Mechanism Of NOS,COX And P38MAPK In Tissues Of Rats Model With FSUI

Objective:The molecular mechanism of FSUI is still unclear. In this study, to explore the mechanism of NOS, COX and p38MAPK in the genesis and development of FSUI, to provide new ideas and potential molecular targets for drug therapy of FSUI.the expression of p38MAPK, COX and NOS in the vagina, bladder neck, urethra and levator ani muscle tissue of FSUI rats were analyzed by the experimental technique of molecular biology.Methods:1.The real-time fluorescence quantitative PCR (Q-PCR) technique was used to detect the expression of NOS, COX and p38MAPK mRNA in the tissues of the vagina, bladder neck, urethra and levator ani muscle in FSUI rats model,which hade five gruops:control group, vaginal distension (VD+NS) group, vaginal distension and ovariectomy (VD+OVX) group, vaginal distension and SMT (VD+SMT) group, vaginal distension and ovariectomy and SMT (VD+OVX+SMT) group.2.The western blot was used to detect the expression of NOS, COX and p38MAPK in the tissues of the vagina, bladder neck, urethra and levator ani muscle in FSUI rats model,which hade five gruops:control group, VD+NS group, VD+OVX group, VD+SMT group, VD+OVX+SMT group.Results:1. In the VD+NS group, VD+OVX+NS group.the expression level of iNOS and COX-2 in transcription and translation were up-regulated compared with control group, the difference was statistically significant(P<0.01). In the VD+SMT group. VD+OVX+SMT group,the expression level of iNOS and COX-2 in transcription and translation were up-regulated compared with control group, the difference was statistically significant(P<0.05). In the VD+SMT group.the expression level of iNOS and COX-2 in transcription and translation were down-regulated compared with VD+NS group, the difference was statistically significant(P<0.01). In the VD+OVX+SMT group,the expression level of iNOS and COX-2 in transcription and translation were down-regulated compared with VD+OVX+NS group, the difference was statistically significant(P<0.05).2.In the VD+NS group, VD+OVX+NS group,the expression level of nNOS and eNOS in transcription and translation were up-regulated compared with control group, the difference was statistically significant(P<0.01). In the VD+SMT group, VD+OVX+SMT group,the expression level of nNOS and eNOS in transcription and translation were up-regulated compared with control group, the difference was statistically significant(P<0.05).But there was no statistically significant difference of of nNOS and eNOS in transcription and translation between before and after injecting SMT(P>0.05).3.In the VD+NS group, VD+OVX+NS group,the expression level of p38MAPK in transcription and translation were no significant change with control group, the difference was not statistically significant(P>0.05). In the VD+SMT group, VD+OVX+SMT group,the expression level of p38MAPK in transcription and translation were no significant change compared with control group, the difference was not statistically significant(P>0.05).But there was no statistically significant difference of of p38MAPK in transcription and translation between before and after injecting SMT(P>0.05).Conclusions:1. NOS, COX and p38MAPK in the vagina, bladder neck, urethra and levator ani muscle tissue of FSUI rats were expressed.2.In the VD+NS group, VD+OVX+NS group,the expression level of iNOS and COX-2 in transcription and translation were up-regulated compared with control group, and after treated with SMT, their expression were down-regulated.The synergistic effect of iNOS and COX-2 may play significant roles in the genesis and development of FSUI.3. In the VD+NS group, VD+OVX+NS group,the expression level of nNOS and eNOS in transcription and translation were up-regulated compared with control group, but after treated with SMT, their expression showed no significant difference.They may play roles in the genesis and development of FSUI.4. The expression level of p38MAPK in transcription and translation had no significant difference between VD+NS group, VD+OVX+NS group and control group, and the results were the same after treated with SMT. The function of p38MAPK in the genesis and development of FSUI may be not so closely related.