Experimental Study Of Antitumor Immunity In Vitro And Vivo Induced By Dendritic Cell Vaccine Which Modified By GM-CSF Gene And Tumor Cell SPC-A1 MRNA

Objectives: Isolating and culturing dendritic cells (DCs) from human peripheralblood to study the specific immunoresponse effect of DCs in vivo and vitro, which modified by granulocyte-macrophage colony-stimulating factor ( GM-CSF ) gene and tumor cells SPC-A1 mRNA.Methods: Getting mRNA from human adenous carcinoma cells of lungSPC-A1 by amplification and transcription in vitro, and then transfecting GM-CSF gene and SPC-A1 mRNA into DCs by lipofectamine. Co-culturing DCs with T lymphocyte after testing surface markers on DCs on flow cytometry . The ability of DCs inducing the anti-tumor immunoresponse was analysised by measuring the exosomatic specific cytolytic against SPC-A1 tumor cells by MTT in vitro, and doing experiment of prophylactic function on naked mouse model in vivo.Results: 1.Mature DCs isolated from human peripheral blood whose specificsurface markers and functional markers are rised relatively aresuccessfully gained(P<0.01).2.The specific cytolytic in vitro and in vivo of T lymphocyte inducedby GM- mRNA -DCs are significantly rised by compared withcontrol(P<0.01).Conclusions: 1. The specificity and intensity of anti-tumor immunoresponse couldbe strengthened by DCs which modified by GM-CSF gene and SPC-A1 mRNA.