Nephroprotective Effect Of Rosiglitazone On Rat Remnant Kidney

Objective To investigate the nephroprotective effect of rosiglitazone on 5/6 nephrectomized rats and explore its mechanism.Methods Chronic renal failure was induced by 5/6 nephrectomy, the nephrectomized rats were randomly allocated to Rosiglitazone treatment group 1(5mg· kg^-1·d^-1 ) (XL group), Rosiglitazone treatment group2 (15mg ·kg^-1·d^-1) (DL group), and control group (NX group) .The rats with sham operation served as control (SHAM group) . Then 5mg/kg Rosiglitazone, 15mg/kg Rosiglitazone were respectively delivered daily by gavage for 8 weeks, 24 hours urinary protein, BUN, serum creatinine(SCr), serum total cholesterol, triglycerides, blood pressure(BP), Angiotensin II(A₂),erdothelin(ET) were measured at the end of the stuffy. Renal pathological changes were evaluated and immunohistochemistry was used to examine the expression of macrophage chemoattractant protein 1 (MCP-1), matrix metalloproteinase-2(MMP-2) and matrix metalloproteinase-9(MMP-9). Renal mesangial collagen fibers were stained with Picric acid-Sirius red. Renal expression of TGF- β1 mRNA and FN mRNA was detected by RT-PCR.Results(1) Compared with the untreated 5/6 nephrectomized group (NX group), blood pressure of Rosiglitazone treatment groups rats were substantially decreased .(p<0.01).(2) Compared with the NX group, 24 hours urinary protein, BUN, serum creatinine(SCr), serum total cholesterol of Rosiglitazone treatment groups rats were substantially decreased, (p<0.05).(3) Compared with the NX group, Angiotensin II(A₂), erdothelin(ET)of Rosiglitazone treatment groups rats were substantially decreased, (p<0.05).(4) Pathohistological study revealed that, compared with the NX group, there...