The Expression And Clinical Significance Of Bcl-2 Protein, P53 Protein And P170 Protein In Acute Leukemia

ObjectiveTo study the expression levels of bcl-2. p53 and p170 in patients with acute leukemia, analyse their relationship with some clinical features, curative effects and prognostic factors of acute leukemia,and explore their clinical significance in acute leukemia. Methods95 patients with acute leukemia were selected as a study group, among which 54 cases were male and 41 cases were female between 16 and 74 years old. All patients were treated by standard chemotherapy scheme. According to their therapeutic outcome, the patients were divided into three groups: 55 cases in untreated group, 21 cases in complete remission group and 19 cases in relapsed/refractory group. 28 healthy persons or patients without hematologic malignancies were selected as a control group. The bone marrow mononuclearcells were isolated by Ficoll-paque, and the bcl-2 protein, p53 protein as well as p170 protein were ditermined by flow cytometry. The data were analysised by SPSS11.5. Results1. The expression rates of bcl-2. p53 and p170 in 95 patients with acute leukemia were49.5%, 42.1% and 69.5%. respectively. The positive rate of bcl-2 of the relapsed/ refractory group was higher than that of the incipient group, and the remission group showed the lowest positive rate among the three groups. The positive rate of p53 of the refractory/relapsed group was higher than those of the incipient group and the remission group, while no difference was observed between the incipient group and the remission group. As for p170, the incipient group showed a lower positive rate than the remission group and the refractory/relapsed group, but no difference was observed between the remission group and the refractory relapsed group. The positive rates of bcl-2, p53 and p170 of the three treatment groups were all higher than of the control group.2. The positive rates of bcl-2. p53 and p170 in ANLL were 46.4%, 51.8% and 75.0%,respectively, while in ALL were 53.8%. 28.2% and 61.5%. respectively. As for the expression levels of bcl-2 and p170. no difference was observed between ANLL and ALL. while the expression level of p53 in ANLL was higher than that in ALL. In acute leukemia patients, no correlation was found between the expression levels of bcl-2, p53 and p170 and the clinical features such as gender, age. peripheral blood leucocyte...