Objective: 1 To study the expression of prepeo-orexin and orexin receptors(OX1R, OX2R) in the hypothalamus in dietary obesity rats. 2 To investigate the relationship between expression of prepeo-orexin in the hypothalamus and HPA axis in dietary obesity rats.Methods: 1.We induced the weaning male Spratue-Dawley rats to be obesity with high fat diet for eight weeks. The weight, Lee's index endo-abdominal fat weight, triglycerides, total cholesterol in serum and fasting blood glucose were detected. Expression of prepeo-orexin, OX1R and OX2R mRNA in the hypothalamus was detected by reverse transcript polyerase chain reaction (RT-PCR). Deteced the OX1R expression in the hypothalamus ARC with immunohistochemistry. 2. Expression of CRH mRNA in the hypothalamus, and prepeo-orexin , OX1R and OX2R mRNA in adrenal was detected by RT-PCR. The concentration of serum corticosterone and 24 hours urine corticosterone were detected by enzyme linked immunosorbent assay. Analyzed the relationship between orexin and HPA axis by Correlation analysisResults: 1. The weight, Lee's index, endo-abdominal fat weight, total...
|