Calcium Ionophore Inducing The Differentiation Of Chronic Myelogenous Leukemia Cells Into Dendritic Cells

Objective: To study the role of calcium ionophore inducing CML cells to differentiate into DCs, to analyze the P210 expression in these DCs and to evaluate the effect of these CML-DCs to activate autologous T cells generating cytotoxic activity against CML cells.Methods: Bone marrow mononuclear cells from CML patients were cultured for 96 hours, the morphologic change of these CML mononuclear cell was observed with inverted microscope and electron microscope and the CD antigens of these CML mononuclear cell were analyzed with flow cytometry; P210 expression in CML mononuclear cells and CML-DCs were measured with Westernblot assay;LDH assay was used to evaluate the effect of CML-DCs generating cytotoxic T lymphocyte (CTL) activity against CML cells.Results: Typical DCs morphology were found under inverted microscope and electron microscope after CML cells are cocultured with calcium ionophore and GM-CSF for 96 h, in the meantime flow cytometry showed that expression of CD83,CD86,CD40,CD80 and HLA-DR increased remarkably;Westernblot assay demonstrated that P210 was expressed both in CML cells and CML-DCs, but the expression level lower in CML-DCs than in CML cells; LDH assay proved raised CTL activity using these CML-DCs to stimulate CML patient's T cells compared with using CML cells.